Manipulative Experiment

Week	Activity	Resources
1	Lab safety, Introduction to Microbiomes, Introduction to Bean Beetles, Experimental Design, Experimental Manipulation, Pre-semester Survey	Introduction to microbiomes powerpoint Introduction to bean beetles powerpoint Instructor's notes on introduction to bean beetles Video tutorial on handling and sexing of bean beetles Suggested readings
2	Other curriculum to allow for beetle development
3	Other curriculum
4	Other curriculum
5	Other curriculum
6	Initial steps of DNA extraction for microbial community sequencing, culturing microbes	Instructor’s notes on DNA extraction Student handout on DNA extraction [docx] [pdf] Video tutorial on DNA extraction DNAeasy Blood & Tissue Insect Protocol DNAeasy Blood & Tissue Handbook Instructor’s notes on preparing media & pouring plates Instructor’s notes on culturing microbial communities Student handout on culturing microbial communities [docx] [pdf] Video tutorial on surface sterilization of bean beetles Video tutorial on culturing microbial communities
7	Phenotypic assessment of microbes, colony-based PCR	Instructor’s notes on culturing microbial communities Student handout on culturing microbial communities [docx] [pdf] Instructor’s notes on colony-based PCR Student handout on colony-based PCR [docx] [pdf] What is PCR? Polymerase Chain Reaction | miniPCR bio™ video Introduction of Sanger Sequencing video Video tutorial on colony-based PCR
8	Electrophoresis and Iteration Day for failed culture-based PCR	Instructor’s notes on electrophoresis Student handout on colony-based PCR [docx] [pdf] What is Gel Electrophoresis? | miniPCR bio™ video Video tutorial on gel electrophoresis
9	Introduction to bioinformatics & analysis of colony sequencing data, introduction to community analysis, phenotype & colony sequencing analysis	Student handout on BLAST analysis of sequencing data [docx] [pdf] Studying microbiomes powerpoint Student handout on community analysis of phenotype (class data) [docx] [pdf] Student handout on community analysis of phenotype (database data) [docx] [pdf] Student handout on community analysis of colony-based sequence (class data) [docx] [pdf] Student handout on community analysis of colony-based sequence (database data) [docx] [pdf] Instructor handout on community analysis
10	Other curriculum to allow for DNA sequencing to be completed
11	Other curriculum
12	Other curriculum
13	Community analysis of sequencing data	BeanBeetleMicrobiome app Tutorial [docx] [pdf]
14	Final presentations, Post-semester survey