These data were collected by students at Morehouse College in Spring 2024. Beetles were offspring reared on blackeye peas(N=6)and adzuki beans (N=7)whose mothers host bean was blackeye peas. Negative control data were subtracted from the sample data. Beetles were from the SI-3 strain adapted to their host bean for many generations prior to eggs laid on either blackeye peas or adzuki beans. Beetles were adults of unknown sex. Beetles were surface-sterilized. Beetles were extracted with QIAGEN DNA-Easy kits. Sequences are for the v4 region. MiSeq sequencing was done by MrDNA labs. Metadata and Sequence Sample ID notation is "Mothers host.Offspring host".