Bean Beetles

Bean Beetles

A Model Organism for Inquiry-based Undergraduate Laboratories

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Bean Beetle Microbiome Methods

Posted on July 14, 2021By cbeck

Below are the standard laboratory methods that we use to study the microbiome of bean beetles.

Culturing of microbes

  • Instructor’s notes on preparing media & pouring plates
  • Instructor’s notes on culturing microbial communities
  • Student handout on culturing microbial communities
  • Video tutorial on surface sterilization of bean beetles
  • Video tutorial on culturing microbial communities

Colony-based PCR

  • Instructor’s notes on colony-based PCR
  • Student handout on colony-based PCR
  • Video tutorial on colony-based PCR
  • Instructor’s notes on electrophoresis
  • Video tutorial on gel electrophoresis

DNA extraction for microbial community sequencing

  • Instructor’s notes on DNA extraction
  • Student handout on DNA extraction
  • Video tutorial on DNA extraction
  • DNAeasy Blood & Tissue Insect Protocol
  • DNAeasy Blood & Tissue Handbook
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