Below are the standard laboratory methods that we use to study the microbiome of bean beetles.
Culturing of microbes
Instructor’s notes
- Instructor’s notes on preparing media & pouring plates
- Instructor’s notes on culturing microbial communities
Student handouts
Video tutorials
- Video tutorial on surface sterilization of bean beetles
- Video tutorial on culturing microbial communities
Colony-based PCR
Instructor’s notes
Student handouts
Video tutorials
DNA extraction for microbial community sequencing
Instructor’s notes
- Instructor’s notes on DNA extraction
- DNAeasy Blood & Tissue Insect Protocol
- DNAeasy Blood & Tissue Handbook
Student handouts
Video tutorials
Oxford Nanopore sequencing
Instructor’s notes
- ONT Pre-evaluating DNA samples
- Preparing for ONT sequencing
- Nanopore Library Preparation and Sequencing
- Sample Pooling Spreadsheet
- Using the Qubit Fluorometer to evaluate dsDNA
- ONT Data Acquisition and Basecalling
- ONT Basecalling and Bioinformatics after Sequencing
Student handouts